Although not a significant wildlife tree, some species benefit from sycamore trees, including songbirds like finches and juncos that eat the seeds. Beaver, squirrel, and muskrat also consume the fruits, and white-tailed deer occasionally browse on the vegetation.

Many cavity-nesting birds - such as owls, flycatchers, and chimney swifts - inhabit the nearly hollow trunks of older trees. Wood ducks are known to build nests in the old tree trunks, and some species of bats use sycamores as nursery trees.